pAd-Track HA PGC-1 alpha
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14427||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9000
Vector typeMammalian Expression, Adenoviral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert namePGC-1 alpha
Alt namePPAR gamma coactivator 1
SpeciesM. musculus (mouse)
Insert Size (bp)2400
MutationLast four amino acids of PGC-1a removed to make the protein more stable.
Entrez GenePpargc1a (a.k.a. A830037N07Rik, Gm11133, PGC-1, PPARGC-1-alpha, Pgc-1alpha, Pgc1, Pgco1, Ppargc1)
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pAdTrack can be recombined with pAdEasy to make adenoviral-PGC1.
See He et al. http://www.pnas.org/cgi/content/full/95/5/2509 for protocols.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAd-Track HA PGC-1 alpha was a gift from Pere Puigserver (Addgene plasmid # 14427 ; http://n2t.net/addgene:14427 ; RRID:Addgene_14427)
For your References section:GCN5 acetyltransferase complex controls glucose metabolism through transcriptional repression of PGC-1alpha. Lerin C, Rodgers JT, Kalume DE, Kim SH, Pandey A, Puigserver P. Cell Metab. 2006 Jun . 3(6):429-38. 10.1016/j.cmet.2006.04.013 PubMed 16753578