PurposeYeast Expression plasmid for SARS-CoV-2 ORF7a protein
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||145483||Standard format: Plasmid sent in bacteria as agar stab||1||$85 *|
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Vector backboneAmpR; 2u URA3
- Backbone size w/o insert (bp) 4078
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameSARS-CoV-2 ORF7a protein
SpeciesS. cerevisiae (budding yeast); Severe acute respiratory syndrome coronavirus 2
Insert Size (bp)359
MutationSaccharomyces cerevisiae recode 1
Entrez GeneORF7a (a.k.a. GU280_gp07)
- Promoter pGAL1
/ Fusion Protein
- Cleavable thrombin;3xFLAG (C terminal on backbone)
- Cloning method Unknown
A portion of this plasmid was derived from a plasmid made byGinkgoBioworks
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
NCBI Reference Sequence: YP_009724395.1Created by gene synthesis with Not1 site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGBW-m4046238 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 145483 ; http://n2t.net/addgene:145483 ; RRID:Addgene_145483)