pMSCV neo WT Akt1
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14549||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepMSCV neo
Backbone manufacturerBD Biosciences
- Backbone size w/o insert (bp) 6500
Vector typeMammalian Expression, Retroviral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneAkt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
/ Fusion Protein
- HA tag
- Cloning method Restriction Enzyme
- 5′ sequencing primer MSCV
- 3′ sequencing primer MSCV rev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byPhilip Tsichlis
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMSCV neo WT Akt1 was a gift from Joan Brugge (Addgene plasmid # 14549 ; http://n2t.net/addgene:14549 ; RRID:Addgene_14549)
For your References section:Distinct roles of Akt1 and Akt2 in regulating cell migration and epithelial-mesenchymal transition. Irie HY, Pearline RV, Grueneberg D, Hsia M, Ravichandran P, Kothari N, Natesan S, Brugge JS. J Cell Biol. 2005 Dec 19. 171(6):1023-34. 10.1083/jcb.200505087 PubMed 16365168