PA1375_pET21a(+)C-tag
(Plasmid
#14580)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14580 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET-21a(+)
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Backbone manufacturerMerck
- Backbone size w/o insert (bp) 5443
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameD-Erythronate-4-phosphate Dehydrogenase
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Alt namePa1375
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Alt namePdxB
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SpeciesPseudomonas aeruginosa
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Insert Size (bp)1140
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GenBank IDNP_250066
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Entrez GenepdxB (a.k.a. PA1375)
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PA1375_pET21a(+)C-tag was a gift from SeWon Suh (Addgene plasmid # 14580 ; http://n2t.net/addgene:14580 ; RRID:Addgene_14580) -
For your References section:
Crystal Structure of d-Erythronate-4-phosphate Dehydrogenase Complexed with NAD. Ha JY, Lee JH, Kim KH, Kim do J, Lee HH, Kim HK, Yoon HJ, Suh SW. J Mol Biol. 2007 Mar 2. 366(4):1294-304. 10.1016/j.jmb.2006.12.038 PubMed 17217963
