Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14826||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneXFIVP / pRK5
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1500
Entrez GeneSMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3)
/ Fusion Protein
- VP16 activation domain (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
Full length Smad3 fused to VP16 activation domain
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XFIVP Smad3 was a gift from Rik Derynck (Addgene plasmid # 14826 ; http://n2t.net/addgene:14826 ; RRID:Addgene_14826)
For your References section:The tumor suppressor Smad4/DPC4 and transcriptional adaptor CBP/p300 are coactivators for smad3 in TGF-beta-induced transcriptional activation. Feng XH, Zhang Y, Wu RY, Derynck R. Genes Dev. 1998 Jul 15. 12(14):2153-63. 10.1101/gad.12.14.2153 PubMed 9679060
Map uploaded by the depositor.