pRK Smad3LC Flag
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14829||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSmad3 LC
SpeciesH. sapiens (human)
Insert Size (bp)837
Mutationamino acids 146-425
Entrez GeneSMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3)
/ Fusion Protein
- Flag (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
See Author's map for cloning details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRK Smad3LC Flag was a gift from Rik Derynck (Addgene plasmid # 14829 ; http://n2t.net/addgene:14829 ; RRID:Addgene_14829)
For your References section:The tumor suppressor Smad4/DPC 4 as a central mediator of Smad function. Zhang Y, Musci T, Derynck R. Curr Biol. 1997 Apr 1. 7(4):270-6. 10.1016/S0960-9822(06)00123-0 PubMed 9094310
Map uploaded by the depositor.