PurposeYeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||148972||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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Vector backboneAmpR; 2u URA3
- Backbone size w/o insert (bp) 4078
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameSARS-CoV-2 RNA-dependent RNA polymerase
SpeciesS. cerevisiae (budding yeast); Severe acute respiratory syndrome coronavirus 2
Insert Size (bp)2795
MutationSaccharomyces cerevisiae recode 1
Entrez GeneORF1ab (a.k.a. GU280_gp01)
- Promoter pTEF1
/ Fusion Protein
- Cleavable thrombin;3xFLAG (C terminal on backbone)
- Cloning method Unknown
A portion of this plasmid was derived from a plasmid made byGinkgoBioworks
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
NCBI Reference Sequence: YP_009725307.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGBW-m4046567 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 148972 ; http://n2t.net/addgene:148972 ; RRID:Addgene_148972)