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pPV230.13
(Plasmid #14902)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14902 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPD95.81
  • Backbone manufacturer
    Andrew Fire
  • Backbone size w/o insert (bp) 4492
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Strongyloides stercoralis transitional endoplasmic reticulum ATPase promoter region
  • Alt name
    era-1 promoter
  • Species
    S. stercoralis (nematode)
  • Insert Size (bp)
    958
  • Mutation
    Ss era-1 promoter region as a HindIII-PstI fragment insterted at 149-1112, Ss era-1 3' UTR as an EcorI-EagI fragment inserted at 2116-2713
  • GenBank ID
    AH015511

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer M13
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPV230.13 was a gift from James Lok (Addgene plasmid # 14902 ; http://n2t.net/addgene:14902 ; RRID:Addgene_14902)
  • For your References section:

    Successful transgenesis of the parasitic nematode Strongyloides stercoralis requires endogenous non-coding control elements. Li X, Massey HC, Nolan TJ, Schad GA, Kraus K, Sundaram M, Lok JB. Int J Parasitol. 2006 May 31. 36(6):671-9. 10.1016/j.ijpara.2005.12.007 PubMed 16500658