|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14909||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAndrew Fire
- Backbone size w/o insert (bp) 4493
Vector typeWorm Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert namestrongyloides stercoralis era-1 3' UTR
Alt nameera-1 3' UTR
SpeciesS. stercoralis (nematode)
Insert Size (bp)598
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EagI (not destroyed)
- 5′ sequencing primer M13 forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Promoterless gfp expression construct with era-1 3' UTR.
See Fire Lab C. elegans Vector Kit 1995 Documetation.
PAPER:Int J Parasitol. 2006 May 31;36(6):671-9. Epub 2006 Feb 7 Successful transgenesis of the parasitic nematode Strongyloides stercoralis requires endogenous non-coding control elements.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAJ02 was a gift from James Lok (Addgene plasmid # 14909 ; http://n2t.net/addgene:14909 ; RRID:Addgene_14909)