Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pAAV-nef-CIAO2-Flp
(Plasmid #149296)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 149296 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flp recombinase
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1275
  • Mutation
    Derived from the mouse codon-optomized FlpO. The SV40 NLS signal first start codon have been removed.
  • Entrez Gene
    flp

Cloning Information

Resource Information

Depositor Comments

The CIAO2 construct replaces the lox66/71 pair used in CIAO with the lox43/44 pair to further decrease leak expression - not used in the original publication. Full name: pAAV-nef-AO-lox43-STOP-lox44-Flp-hGH-pA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-nef-CIAO2-Flp was a gift from Edward Callaway (Addgene plasmid # 149296 ; http://n2t.net/addgene:149296 ; RRID:Addgene_149296)
  • For your References section:

    Sources of off-target expression from recombinase-dependent AAV vectors and mitigation with cross-over insensitive ATG-out vectors. Fischer KB, Collins HK, Callaway EM. Proc Natl Acad Sci U S A. 2019 Dec 16. pii: 1915974116. doi: 10.1073/pnas.1915974116. 10.1073/pnas.1915974116 PubMed 31843925