Prham-tagRFP-IMBB2.4-pUC57-mini
(Plasmid #149343)

• Purpose: expresses tagRFP fluorescent protein in E. coli
• Depositing Lab: Ichiro Matsumura
• Publication: Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717/peerj.9841. eCollection 2020.

Backbone

• Vector backbone: pUC
• Backbone manufacturer: GenScript
• Backbone size w/o insert (bp): 1632
• Total vector size (bp): 2939
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Please use Mach1 bacterial strain for expression.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: tagRFP
• Alt name: Red Fluorescent Protein
• Species: Synthetic; Entacmaea quadricolor
• Insert Size (bp): 694
• GenBank ID: KJ908188
• Promoter: rhamnose

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: part sequenced in different plasmid prior to subcloning
• 3′ sequencing primer: part sequenced in different plasmid prior to subcloning

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry