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Methylase-assisted subcloning for high throughput BioBrick assembly.
Matsumura I
PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717/peerj.9841. eCollection 2020.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
149338Prham-M.XbaI-p15A-aadAexpresses M.XbaI DNA methyltransferase in E. coli
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149339Prham-M.Ocy1ORF8430P-p15A-aadAexpresses M.Ocy1ORF8430P DNA methyltransferase (M.SpeI ortholog) in E. coli
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149340Prham-M.PstI-p15A-aadAexpresses M.PstI DNA methyltransferase in E. coli
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149341Prham-M.EcoRI-p15A-aadAexpresses M.EcoRI DNA methyltransferase in E. coli
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149342Prham-M.AvaIII-p15A-aadAexpresses M.AvaIII DNA methyltransferase (M.NsiI ortholog) in E. coli
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149343Prham-tagRFP-IMBB2.4-pUC57-miniexpresses tagRFP fluorescent protein in E. coli
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Antibodies from Article