Prham-M.EcoRI-p15A-aadA
(Plasmid #149341)

• Purpose: expresses M.EcoRI DNA methyltransferase in E. coli
• Depositing Lab: Ichiro Matsumura
• Publication: Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717/peerj.9841. eCollection 2020.

Backbone

• Vector backbone: pACYC
• Backbone manufacturer: EMD Millipore/Novagen
• Backbone size w/o insert (bp): 1724
• Total vector size (bp): 3352
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Please use OmniMax 2 bacterial strain for expression.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: M.EcoRI
• Alt name: EcoRI DNA methyltransferase
• Species: Synthetic; Plasmid RI13 (from E.coli)
• GenBank ID: J01675
• Promoter: rhamnose

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (destroyed during cloning)
• 3′ cloning site: SpeI (destroyed during cloning)
• 5′ sequencing primer: part sequenced in different plasmid prior to subcloning
• 3′ sequencing primer: part sequenced in different plasmid prior to subcloning

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry