pCMV-UnaG-pA (pE091)
(Plasmid
#149354)
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PurposeMammalian Expression vector for UnaG
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Depositing Lab
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Depositing OrganizationMax-Delbrueck-Centrum fuer Molekulare Medizin (MDC)
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 149354 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 149354-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 149354-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepuc19
- Backbone size w/o insert (bp) 2218
- Total vector size (bp) 4934
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUnaG
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Insert Size (bp)420
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer M13 for
- 3′ sequencing primer M13 rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 149354-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 149354-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-UnaG-pA (pE091) was a gift from Ralf Kuehn (Addgene plasmid # 149354 ; http://n2t.net/addgene:149354 ; RRID:Addgene_149354) -
For your References section:
A homology independent sequence replacement strategy in human cells using a CRISPR nuclease. Danner E, Lebedin M, de la Rosa K, Kuhn R. Open Biol. 2021 Jan;11(1):200283. doi: 10.1098/rsob.200283. Epub 2021 Jan 27. 10.1098/rsob.200283 PubMed 33499763
Map uploaded by the depositor.
