PurposeBacterial expression plasmid for SARS-CoV-2 nsp3
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||149525||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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Vector backboneori-013 - marker | cmR
- Backbone size w/o insert (bp) 1462
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
Copy numberHigh Copy
Gene/Insert nameSARS-CoV-2 nsp3
SpeciesEscherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Insert Size (bp)341
Entrez GeneORF1ab (a.k.a. GU280_gp01)
- Promoter Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ; Start Codon | ATG
/ Fusion Protein
- Cleavable TEV;6xHIS (C terminal on backbone)
- Cloning method Unknown
A portion of this plasmid was derived from a plasmid made byGinkgoBioworks
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
NCBI reference sequence: YP_009725299.1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGBW-m4095418 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 149525 ; http://n2t.net/addgene:149525 ; RRID:Addgene_149525)