pCMV5 Flag-Smad3 (220-425)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14967||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)618
Entrez GeneSMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV5 Flag-Smad3 (220-425) was a gift from Joan Massague (Addgene plasmid # 14967 ; http://n2t.net/addgene:14967 ; RRID:Addgene_14967)
For your References section:E2F4/5 and p107 as Smad cofactors linking the TGFbeta receptor to c-myc repression. Chen CR, Kang Y, Siegel PM, Massague J. Cell. 2002 Jul 12. 110(1):19-32. 10.1016/S0092-8674(02)00801-2 PubMed 12150994
Map uploaded by the depositor.