Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15094||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6470
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameTRAF6 3'UTR
SpeciesH. sapiens (human)
Insert Size (bp)500
Mutation4-bp substitutions in the miR-146 target sites
Entrez GeneTRAF6 (a.k.a. MGC:3310, RNF85)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site MluI (not destroyed)
- 5′ sequencing primer Luc-F
- 3′ sequencing primer M13-F20 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Mutated version of luciferase reporter construct containing ~500 bp of 3' UTR of TRAF6 with 4-bp substitutions in the miR-146 target sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:TRAF6mut-UTR was a gift from David Baltimore (Addgene plasmid # 15094 ; http://n2t.net/addgene:15094 ; RRID:Addgene_15094)
For your References section:NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses. Taganov KD, Boldin MP, Chang KJ, Baltimore D. Proc Natl Acad Sci U S A. 2006 Aug 15. 103(33):12481-6. 10.1073/pnas.0605298103 PubMed 16885212