pTBNde(min)
(Plasmid #15125)

• Depositing Lab: Franco Pagani
• Publication: Pagani et al Hum Mol Genet. 2003 May 15. 12(10):1111-20.

Backbone

• Vector backbone: pBlueScript II KS
• Backbone size w/o insert (bp): 2960
• Modifications to backbone: modified to include an alpha-globin-fibronectin EDB minigene in which transcription is driven from the minimal α-globin promoter and SV40 enhancer
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: alpha-globin EDB fibronectin minigene
• Species: H. sapiens (human)
• Insert Size (bp): 3151
• Entrez Gene: FN1 (a.k.a. CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF)
• Promoter: alpha-globin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: M13 DIR
• 3′ sequencing primer: M13 REV

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Hybrid minigene used for splicing assay for pre-mRNA processing. Contains a unique NdeI site for subcloning sequence of interest.