Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pSLIRES-11 (CC#13)
(Plasmid #15145)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15145 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSLIRES-11
  • Backbone manufacturer
    Cepko Lab
  • Backbone size (bp) 3552
  • Vector type
    Adapter vector for cloning into pRISAP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • IRES (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

In pSLIRES11, the 11th ATG of the IRES was modified to an NcoI site. The transgene can be introduced into pSLIRES11 by changing its initial methionine codon ATG to an NcoI site. The IRES and the inserted cDNA fragment can be excised as a single fragment using ClaI digestion and can then be cloned into the unique ClaI site of pRISAP. As a result both the PLAP marker and the transgene will be encoded by a spliced bicistronic message. In addition, the gene under the control of the IRES can be translated from the full-length unspliced mRNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSLIRES-11 (CC#13) was a gift from Connie Cepko (Addgene plasmid # 15145 ; http://n2t.net/addgene:15145 ; RRID:Addgene_15145)
  • For your References section:

    Production and design of more effective avian replication-incompetent retroviral vectors. Chen CM, Smith DM, Peters MA, Samson ME, Zitz J, Tabin CJ, Cepko CL. Dev Biol. 1999 Oct 15. 214(2):370-84. 10.1006/dbio.1999.9432 PubMed 10525341