Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

DAP (DOL-AP) (CC#44)
(Plasmid #15164)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15164 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDOL
  • Vector type
    Mammalian Expression, Retroviral ; Avian expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PLAP
  • Alt name
    placental alkaline phosphatase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Entrez Gene
    ALPP (a.k.a. ALP, ALPI, IAP, PALP, PLAP, PLAP-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pDOL was a gift from Dr. Richard Mulligan. PLAP was a gift from Dr. John Flanagan and Dr. Philip Leder.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Retroviral vector expressing PALP from the long-terminal-repeat (LTR) promoter. The neomycin gene is expressed from SV40 promoter.

Plasmid CMV-PLAP was digested with KpnI and a 1930bp fragment encoding PLAP was isolated. The ends of this fragment were blunted with T4 DNA polymerase and ligated to a SalI linker. The linker fragment was digested with SalI and inserted into the SalI site of the Moloney murine leukemia virus retroviral vector pDOL.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DAP (DOL-AP) (CC#44) was a gift from Connie Cepko (Addgene plasmid # 15164 ; http://n2t.net/addgene:15164 ; RRID:Addgene_15164)
  • For your References section:

    A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina. Fields-Berry SC, Halliday AL, Cepko CL. Proc Natl Acad Sci U S A. 1992 Jan 15. 89(2):693-7. 10.1073/pnas.89.2.693 PubMed 1731342