PurposeMammalian expression plasmid for SARS-CoV-2 nsp8
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||152258||Standard format: Plasmid sent in bacteria as agar stab||1||$85 *|
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- Backbone size w/o insert (bp) 6080
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameSARS-CoV-2 nsp8
SpeciesSevere acute respiratory syndrome coronavirus 2
Insert Size (bp)587
Entrez GeneORF1ab (a.k.a. GU280_gp01)
- Promoter CMV
/ Fusion Protein
- Cleavable TEV;HA (C terminal on backbone)
- Cloning method Unknown
A portion of this plasmid was derived from a plasmid made byGinkgoBioworks
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
NCBI reference sequence: YP_009725304.1
Please note Addgene NGS result found a single mismatch in the SV40 poly A signal compared to Ginkgo BioWorks deposited sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGBW-m4133908 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 152258 ; http://n2t.net/addgene:152258 ; RRID:Addgene_152258)