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pMKO.1-Puro-shPP2A-Aalpha
(Plasmid #15249)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 15249 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMKO.1
  • Backbone size w/o insert (bp) 6700
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PP2A regulatory subunit A, alpha isoform
  • Alt name
    PP2A A alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    58
  • Entrez Gene
    PPP2R1A (a.k.a. MRD36, PP2A-Aalpha, PP2AA, PP2AAALPHA, PR65A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN 5'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Target sequence: GACAACAGCACCTTGCAGAGT

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMKO.1-Puro-shPP2A-Aalpha was a gift from William Hahn (Addgene plasmid # 15249 ; http://n2t.net/addgene:15249 ; RRID:Addgene_15249)
  • For your References section:

    The tumor suppressor PP2A Abeta regulates the RalA GTPase. Sablina AA, Chen W, Arroyo JD, Corral L, Hector M, Bulmer SE, DeCaprio JA, Hahn WC. Cell. 2007 Jun 1. 129(5):969-82. 10.1016/j.cell.2007.03.047 PubMed 17540176