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pMKO.1 GFP B55 alpha shRNA 3-1
(Plasmid #10679)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 10679 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6700
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    PP2A B55 alpha shRNA
  • Alt name
    PP2A B55 alpha
  • Alt name
    B55 alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    PPP2R2A (a.k.a. B55A, B55ALPHA, PR52A, PR55A, PR55alpha)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN 5'
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To make retroviruses expressing shRNAs, oligonucleotides were inserted in the vector pMKO.1-puro modified by replacing the puromycin resistance gene with hrGFP (pMKO.1-GFP). The shRNAs were generated by cloning nucleotides 1156-1175 of PP2A B55 alpha followed by a 6 bp loop and the corresponding antisense sequence followed by five thymidines into pMKO.1-GFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMKO.1 GFP B55 alpha shRNA 3-1 was a gift from William Hahn (Addgene plasmid # 10679 ; ; RRID:Addgene_10679)
  • For your References section:

    Identification of specific PP2A complexes involved in human cell transformation. Chen W, Possemato R, Campbell KT, Plattner CA, Pallas DC, Hahn WC. Cancer Cell. 2004 Feb . 5(2):127-36. 10.1016/S1535-6108(04)00026-1 PubMed 14998489