pMKO.1 GFP B55 alpha shRNA 3-1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10679||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6700
Vector typeMammalian Expression, Retroviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePP2A B55 alpha shRNA
Alt namePP2A B55 alpha
Alt nameB55 alpha
SpeciesH. sapiens (human)
Insert Size (bp)58
Entrez GenePPP2R2A (a.k.a. B55A, B55ALPHA, PR52A, PR55A, PR55alpha)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
To make retroviruses expressing shRNAs, oligonucleotides were inserted in the vector pMKO.1-puro modified by replacing the puromycin resistance gene with hrGFP (pMKO.1-GFP). The shRNAs were generated by cloning nucleotides 1156-1175 of PP2A B55 alpha followed by a 6 bp loop and the corresponding antisense sequence followed by five thymidines into pMKO.1-GFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMKO.1 GFP B55 alpha shRNA 3-1 was a gift from William Hahn (Addgene plasmid # 10679 ; http://n2t.net/addgene:10679 ; RRID:Addgene_10679)
For your References section:Identification of specific PP2A complexes involved in human cell transformation. Chen W, Possemato R, Campbell KT, Plattner CA, Pallas DC, Hahn WC. Cancer Cell. 2004 Feb . 5(2):127-36. 10.1016/S1535-6108(04)00026-1 PubMed 14998489
Map uploaded by Addgene staff.
Map uploaded by the depositor.