PurposeMammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||152890||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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- Backbone size w/o insert (bp) 4180
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameSARS-CoV-2 RNA-dependent RNA polymerase
SpeciesSevere acute respiratory syndrome coronavirus 2
Insert Size (bp)2795
Entrez GeneORF1ab (a.k.a. GU280_gp01)
- Promoter CMV
/ Fusion Protein
- Cleavable TEV;3xFlag (C terminal on backbone)
- Cloning method Unknown
A portion of this plasmid was derived from a plasmid made byGinkgoBioworks
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
NCBI reference sequence: YP_009725307.1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGBW-m4134347 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 152890 ; http://n2t.net/addgene:152890 ; RRID:Addgene_152890)