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LARS_pNIC-Bio3
(Plasmid #153052)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 153052 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pNIC-Bio3
  • Backbone manufacturer
    Structural Genomics Consortium
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LARS
  • Species
    H. sapiens (human)
  • Mutation
    Not full length protein
  • Entrez Gene
    LARS1 (a.k.a. HSPC192, ILFS1, LARS, LEURS, LEUS, LFIS, LRS, PIG44, RNTLS, hr025Cl)
  • Promoter T7
  • Tags / Fusion Proteins
    • His6-TEV (N terminal on backbone)
    • Avi (C terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Backbone vector pNIC-Bio3, Genbank acc. no. JN792439. Please note that this plasmid may not encode full length LARS. Please see the plasmid sequences for additional information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LARS_pNIC-Bio3 was a gift from Susanne Gräslund (Addgene plasmid # 153052 ; http://n2t.net/addgene:153052 ; RRID:Addgene_153052)