-
Depositing Lab
-
Depositing OrganizationUniversity of Washington
Located in United States of America -
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15311 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 15311-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 15311-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backboneZem3 (pUC13 + MT prom + hGH polyA)
-
Backbone manufacturerDr. Richard Palmiter
- Backbone size w/o insert (bp) 4100
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePRKACB
-
Alt namecAMP-dependent protein kinase catalytic subunit beta
-
Alt namePKACB
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1246
-
Entrez GenePrkacb (a.k.a. CbPKA, Pkacb)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Zem3 is a pUC13 derivative that contains a mouse metallothionein-I promoter and the human growth hormone pol(A) region separated by a BglII restriction site. pCbeta EV was constructed by isolating the 1246bp SacI fragment of pMCbeta and inserting it into the BglII site of Zem3 after ligation of BamHI linkers onto the cDNA fragment.
DNA (Catalog # 15311-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 15311-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCbeta EV (PKA catalytic subunit Cbeta) was a gift from Stanley McKnight (Addgene plasmid # 15311 ; http://n2t.net/addgene:15311 ; RRID:Addgene_15311) -
For your References section:
Expression of cDNAs for two isoforms of the catalytic subunit of cAMP-dependent protein kinase. Uhler MD, McKnight GS. J Biol Chem. 1987 Nov 5. 262(31):15202-7. PubMed 3667630
Map uploaded by the depositor.
