pT2-CMV/TetO2-EYFP-GW
(Plasmid
#153309)
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Purpose(Empty Backbone) Tet-On YFP-tagged Gateway destination vector, to be used together with pT2-TetR-neoR transposon plasmid
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153309 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepT2/HB
- Backbone size (bp) 3558
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Vector typeTransposon
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Tag
/ Fusion Protein
- Yellow Fluorescent Protein (N terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT2-CMV/TetO2-EYFP-GW was a gift from Spyros Petrakis (Addgene plasmid # 153309 ; http://n2t.net/addgene:153309 ; RRID:Addgene_153309) -
For your References section:
Nuclear inclusions of pathogenic ataxin-1 induce oxidative stress and perturb the protein synthesis machinery. Laidou S, Alanis-Lobato G, Pribyl J, Rasko T, Tichy B, Mikulasek K, Tsagiopoulou M, Oppelt J, Kastrinaki G, Lefaki M, Singh M, Zink A, Chondrogianni N, Psomopoulos F, Prigione A, Ivics Z, Pospisilova S, Skladal P, Izsvak Z, Andrade-Navarro MA, Petrakis S. Redox Biol. 2020 Feb 11;32:101458. doi: 10.1016/j.redox.2020.101458. 10.1016/j.redox.2020.101458 PubMed 32145456
