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FLAG-Med9 pBacPAK8
(Plasmid #15363)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15363 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBacPAK8
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5538
  • Vector type
    Baculovirus construction

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Med9
  • Alt name
    Med25
  • Alt name
    FLJ10193
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    430
  • Entrez Gene
    Med9 (a.k.a. Med25, BC019367, FLJ10193, MGC30366)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1/Nhe1 (destroyed during cloning)
  • 3′ cloning site Kpn1 (not destroyed)
  • 5′ sequencing primer Bac1
  • 3′ sequencing primer Bac2
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-Med9 pBacPAK8 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15363 ; http://n2t.net/addgene:15363 ; RRID:Addgene_15363)
  • For your References section:

    A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW. J Biol Chem. 2004 Feb 13. 279(7):5846-51. 10.1074/jbc.M312523200 PubMed 14638676