PurposeMammalian expression plasmid for SARS-CoV-2 nsp11
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||153774||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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Vector backbonepTwist_CMV_Hygro_6 with AarI site removed
- Backbone size w/o insert (bp) 6525
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameSARS-CoV-2 nsp11
SpeciesSevere acute respiratory syndrome coronavirus 2
Insert Size (bp)32
Entrez GeneORF1ab (a.k.a. GU280_gp01)
- Promoter CMV
/ Fusion Protein
- Cleavable TEV;TRX (C terminal on backbone)
- Cloning method Unknown
A portion of this plasmid was derived from a plasmid made byGinkgoBioworks
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
NCBI reference sequence: 43740578
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGBW-m4252897 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 153774 ; http://n2t.net/addgene:153774 ; RRID:Addgene_153774)