LB322 (Bcl-2 from ATG to -3934)
(Plasmid #15381)

• Depositing Lab: Linda Boxer
• Publication: Heckman et al J Biol Chem. 2000 Mar 3. 275(9):6499-508.

Backbone

• Vector backbone: pBluescript II KS(+)
• Backbone size w/o insert (bp): 6000
• Vector type: Bacterial Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: B-cell lymphoma/leukemia-2
• Alt name: Bcl-2
• Species: H. sapiens (human)
• Insert Size (bp): 3926
• Mutation: Bcl-2 fragment from ATG to -3934 with both P1 and P2
• Entrez Gene: BCL2 (a.k.a. Bcl-2, PPP1R50)
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry