LB595 (Bcl-2 promoter CRE mut)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15243||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBSII KS+
- Backbone size w/o insert (bp) 2961
Vector typeBacterial Expression, Luciferase
Growth in Bacteria
Gene/Insert nameB-cell lymphoma/leukemia-2
SpeciesH. sapiens (human)
Insert Size (bp)354
MutationMutated CRE site at -1537 changed from GTGACGTTA to GGGCCTTTA
Entrez GeneBCL2 (a.k.a. Bcl-2, PPP1R50)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SacII (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LB595 (Bcl-2 promoter CRE mut) was a gift from Linda Boxer (Addgene plasmid # 15243 ; http://n2t.net/addgene:15243 ; RRID:Addgene_15243)
For your References section:NF-kappaB activates Bcl-2 expression in t(14;18) lymphoma cells. Heckman CA, Mehew JW, Boxer LM. Oncogene. 2002 May 30. 21(24):3898-908. 10.1038/sj.onc.1205483 PubMed 12032828