LB1366 (Bcl-2 promoter with MHS1234)
(Plasmid
#15388)
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Depositing Lab
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Depositing OrganizationStanford University
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15388 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 15388-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 15388-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepREP4
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 10183
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Vector typeMammalian Expression, Bacterial Expression, Luciferase
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameB-cell lymphoma/leukemia-2
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Alt nameBcl-2
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SpeciesH. sapiens (human)
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Insert Size (bp)4125
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MutationBcl-2 full promoter with IgH full enhancer MHS1234
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Entrez GeneBCL2 (a.k.a. Bcl-2, PPP1R50)
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Tag
/ Fusion Protein
- Luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer pREP_fwd
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 15388-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 15388-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LB1366 (Bcl-2 promoter with MHS1234) was a gift from Linda Boxer (Addgene plasmid # 15388 ; http://n2t.net/addgene:15388 ; RRID:Addgene_15388) -
For your References section:
The immunoglobulin heavy-chain gene 3' enhancers deregulate bcl-2 promoter usage in t(14;18) lymphoma cells. Duan H, Heckman CA, Boxer LM. Oncogene. 2007 Apr 19. 26(18):2635-41. 10.1038/sj.onc.1210061 PubMed 17043638
Map uploaded by the depositor.
