pDD2148
(Plasmid
#153968)
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PurposepCMV6-Entry vector encoding human IRAK1 protein 196F
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Depositing Lab
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Depositing OrganizationUniversity of North Carolina at Chapel Hill
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153968 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 153968-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 153968-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCMV6-Entry
- Backbone size w/o insert (bp) 4500
- Total vector size (bp) 7500
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Modifications to backboneIRAK1 position 196 was changed from S196F
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Vector typeMammalian Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIRAK1
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SpeciesH. sapiens (human)
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Insert Size (bp)2800
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GenBank IDNM_001569
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Entrez GeneIRAK1 (a.k.a. IRAK, pelle)
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Tag
/ Fusion Protein
- Myc-DDK
Resource Information
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A portion of this plasmid was derived from a plasmid made byWe purchased the original plasmid from Origene CAT#: RC221544 and had blue heron modify it so that IRAK1 is 196F
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 153968-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 153968-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDD2148 was a gift from Dirk Dittmer (Addgene plasmid # 153968 ; http://n2t.net/addgene:153968 ; RRID:Addgene_153968) -
For your References section:
Interleukin-1 Receptor-Associated Kinase (IRAK) Signaling in Kaposi Sarcoma-Associated Herpesvirus-Induced Primary Effusion Lymphoma. Seltzer J, Moorad R, Schifano JM, Landis JT, Dittmer DP. J Virol. 2020 May 4;94(10). pii: JVI.02123-19. doi: 10.1128/JVI.02123-19. Print 2020 May 4. 10.1128/JVI.02123-19 PubMed 32161170
Map uploaded by the depositor.
