Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pDD2148
(Plasmid #153968)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 153968 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV6-Entry
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 7500
  • Modifications to backbone
    IRAK1 position 196 was changed from S196F
  • Vector type
    Mammalian Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IRAK1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2800
  • GenBank ID
    NM_001569
  • Entrez Gene
    IRAK1 (a.k.a. IRAK, pelle)
  • Tag / Fusion Protein
    • Myc-DDK

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We purchased the original plasmid from Origene CAT#: RC221544 and had blue heron modify it so that IRAK1 is 196F

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDD2148 was a gift from Dirk Dittmer (Addgene plasmid # 153968 ; http://n2t.net/addgene:153968 ; RRID:Addgene_153968)
  • For your References section:

    Interleukin-1 Receptor-Associated Kinase (IRAK) Signaling in Kaposi Sarcoma-Associated Herpesvirus-Induced Primary Effusion Lymphoma. Seltzer J, Moorad R, Schifano JM, Landis JT, Dittmer DP. J Virol. 2020 May 4;94(10). pii: JVI.02123-19. doi: 10.1128/JVI.02123-19. Print 2020 May 4. 10.1128/JVI.02123-19 PubMed 32161170