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(Plasmid #154281)


Item Catalog # Description Quantity Price (USD)
Plasmid 154281 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Campeau et al. PLOS One 2009
  • Backbone size w/o insert (bp) 2300
  • Total vector size (bp) 5384
  • Vector type
    Gateway Entry Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy


  • Gene/Insert name
    O-GlcNAc Transferase
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    WT OGT
  • GenBank ID
  • Entrez Gene
    OGT (a.k.a. HINCUT-1, HRNT1, MRX106, O-GLCNAC, OGT1, XLID106)
  • Tags / Fusion Proteins
    • mKate2 (N terminal on insert)
    • 2x FLAG (N terminal on insert)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR1A hu_ncOGT WT was a gift from Suzanne Walker (Addgene plasmid # 154281 ; ; RRID:Addgene_154281)
  • For your References section:

    Mammalian cell proliferation requires noncatalytic functions of O-GlcNAc transferase. Levine ZG, Potter SC, Joiner CM, Fei GQ, Nabet B, Sonnett M, Zachara NE, Gray NS, Paulo JA, Walker S. Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2016778118. doi: 10.1073/pnas.2016778118. 10.1073/pnas.2016778118 PubMed 33419956