Mammalian cell proliferation requires noncatalytic functions of O-GlcNAc transferase.
Levine ZG, Potter SC, Joiner CM, Fei GQ, Nabet B, Sonnett M, Zachara NE, Gray NS, Paulo JA, Walker S
Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2016778118. doi: 10.1073/pnas.2016778118. PubMed Article
Levine ZG, Potter SC, Joiner CM, Fei GQ, Nabet B, Sonnett M, Zachara NE, Gray NS, Paulo JA, Walker S
Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2016778118. doi: 10.1073/pnas.2016778118. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154281 | pENTR1A hu_ncOGT WT | Entry vector encoding non-tagged O-GlcNAc Transferase |
| 154282 | pENTR1A mKate2-hu_ncOGT WT | Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; WT) |
| 154283 | pENTR1A mKate2-hu_ncOGT D554N | Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation) |
| 154284 | pENTR1A mKate2-hu_ncOGT K842M | Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead) |
| 154285 | pENTR1A mKate2-hu_ncOGT 5N5A | Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage) |
| 154287 | pENTR1A FKBP12F36V-OGT | Entry vector encoding FKBP12(F36V)-2xHA-OGT (dTAG fused-O-GlcNAc Transferase) |
| 154289 | pLenti PGK OGT (Neo) | Lentiviral vector encoding full length wildtype human OGT |
| 154290 | pLenti PGK mKate2-OGT WT (Puro) | Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, WT) |
| 154291 | pLenti PGK mKate2-OGT K842M (Puro) | Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, K842M variant, catalytic dead) |
| 154292 | pLenti PGK mKate2-OGT D554N (Puro) | Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation) |
| 154293 | pLenti PGK mKate2-OGT 5N5A (Puro) | Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage) |
| 154294 | pLenti PGK FKBP12(F36V)-OGT (Neo) | Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoter |
| 155196 | pLEX_305-N-dTAG_OGT | N-terminal FKBP12(F36V)-2xHA tag on lentiviral OGT construct |
| 155197 | pENTR1A hu_ncOGT WT (open N-term) | Entry vector encoding non-tagged O-GlcNAc Transferase with open N-terminus |