-227 hTF (mutant AP-1)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15444||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepGL2 Basic
- Backbone size w/o insert (bp) 5600
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameTissue factor promoter
SpeciesH. sapiens (human)
Insert Size (bp)348
MutationBoth AP-1 sites mutated
Entrez GeneF3 (a.k.a. CD142, TF, TFA)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer na
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
-227 to +121 hTF promoter with both AP-1 sites mutated. hTF promoter deletion series, Mackman lab #2586.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:-227 hTF (mutant AP-1) was a gift from Nigel Mackman (Addgene plasmid # 15444 ; http://n2t.net/addgene:15444 ; RRID:Addgene_15444)
For your References section:Regulation of the tissue factor gene in human monocytic cells. Role of AP-1, NF-kappa B/Rel, and Sp1 proteins in uninduced and lipopolysaccharide-induced expression. Oeth P, Parry GC, Mackman N. Arterioscler Thromb Vasc Biol. 1997 Feb . 17(2):365-74. 10.1161/01.ATV.17.2.365 PubMed 9081693