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-227 hTF (mutant AP-1)
(Plasmid #15444)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15444 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL2 Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tissue factor promoter
  • Alt name
    TF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    348
  • Mutation
    Both AP-1 sites mutated
  • Entrez Gene
    F3 (a.k.a. CD142, TF, TFA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

-227 to +121 hTF promoter with both AP-1 sites mutated. hTF promoter deletion series, Mackman lab #2586.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    -227 hTF (mutant AP-1) was a gift from Nigel Mackman (Addgene plasmid # 15444 ; http://n2t.net/addgene:15444 ; RRID:Addgene_15444)
  • For your References section:

    Regulation of the tissue factor gene in human monocytic cells. Role of AP-1, NF-kappa B/Rel, and Sp1 proteins in uninduced and lipopolysaccharide-induced expression. Oeth P, Parry GC, Mackman N. Arterioscler Thromb Vasc Biol. 1997 Feb . 17(2):365-74. 10.1161/01.ATV.17.2.365 PubMed 9081693