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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15455 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC8
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 2665
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Vector typeBacterial Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTissue factor
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Alt nameTF
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SpeciesH. sapiens (human)
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Insert Size (bp)641
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Entrez GeneF3 (a.k.a. CD142, TF, TFA)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer M13R
- 3′ sequencing primer M13-F20 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mackman lab pCTF 314.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
human TF cDNA was a gift from Nigel Mackman (Addgene plasmid # 15455 ; http://n2t.net/addgene:15455 ; RRID:Addgene_15455) -
For your References section:
Regulation of the tissue factor gene. Mackman N. FASEB J. 1995 Jul . 9(10):883-9. PubMed 7615158