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PSD-95 FLAG
(Plasmid #15463)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15463 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5137
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PSD-95
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2174
  • GenBank ID
    M96853
  • Entrez Gene
    Dlg4 (a.k.a. Dlgh4, PSD95, Sap90)
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer BGH rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Sequence discrepancies should have no functional consequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PSD-95 FLAG was a gift from Wei-dong Yao (Addgene plasmid # 15463 ; http://n2t.net/addgene:15463 ; RRID:Addgene_15463)
  • For your References section:

    Inhibition of the dopamine D1 receptor signaling by PSD-95. Zhang J, Vinuela A, Neely MH, Hallett PJ, Grant SG, Miller GM, Isacson O, Caron MG, Yao WD. J Biol Chem. 2007 May 25. 282(21):15778-89. 10.1074/jbc.M611485200 PubMed 17369255