|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15463||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5137
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesR. norvegicus (rat)
Insert Size (bp)2174
Entrez GeneDlg4 (a.k.a. Dlgh4, PSD95, Sap90)
/ Fusion Protein
- FLAG (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
Sequence discrepancies should have no functional consequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PSD-95 FLAG was a gift from Wei-dong Yao (Addgene plasmid # 15463 ; http://n2t.net/addgene:15463 ; RRID:Addgene_15463)
For your References section:Inhibition of the dopamine D1 receptor signaling by PSD-95. Zhang J, Vinuela A, Neely MH, Hallett PJ, Grant SG, Miller GM, Isacson O, Caron MG, Yao WD. J Biol Chem. 2007 May 25. 282(21):15778-89. 10.1074/jbc.M611485200 PubMed 17369255