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pAS_4xG1 PylT FLAG-G1 PylRS
(Plasmid #154768)


Item Catalog # Description Quantity Price (USD)
Plasmid 154768 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
    G1 PylRS
  • Alt name
  • Species
    methanogenic archaeon mixed culture ISO4-G1
  • Insert Size (bp)
  • Entrez Gene
    MJ_RS07805 (a.k.a. MJ_RS07805, MJ1460, MJ_1460)
  • Promoter EF1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS_4xG1 PylT FLAG-G1 PylRS was a gift from Simon Elsaesser (Addgene plasmid # 154768 ; ; RRID:Addgene_154768)
  • For your References section:

    Site-Specific Incorporation of Two ncAAs for Two-Color Bioorthogonal Labeling and Crosslinking of Proteins on Live Mammalian Cells. Meineke B, Heimgartner J, Eirich J, Landreh M, Elsasser SJ. Cell Rep. 2020 Jun 23;31(12):107811. doi: 10.1016/j.celrep.2020.107811. 10.1016/j.celrep.2020.107811 PubMed 32579937