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Site-Specific Incorporation of Two ncAAs for Two-Color Bioorthogonal Labeling and Crosslinking of Proteins on Live Mammalian Cells.
Meineke B, Heimgartner J, Eirich J, Landreh M, Elsasser SJ
Cell Rep. 2020 Jun 23;31(12):107811. doi: 10.1016/j.celrep.2020.107811. PubMed Article

Plasmids from Article

ID Plasmid Purpose
154763pAS_FLAG-G1 PylRSplasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration
154767pAS_4xG1 PylT sfGFP 150TAGplasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
154768pAS_4xG1 PylT FLAG-G1 PylRSplasmid with 4xG1 PylT cassette and G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration
154769pAS_4xG1 PylT FLAG-G1 PylRS Y125Aplasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration
154770pAS_4xG1 PylT A41AA C55A sfGFP 150TAGplasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
154771pAS_4xhyb PylT sfGFP 150TAGplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
154772pAS_4xhyb PylT A41AA C55A sfGFP 150TAGplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
154773pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125Aplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration
154774pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRSplasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integration
154775pAS_4xU6-PylT M15 (UUA) sfGFP 150TAAplasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration
154776pAS_4xhyb PylT A41AA C55A sfGFP 102TAG 150TAAplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAG 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration
154777pAS_4xhyb PylT A41AA C55A sfGFP 102TAA 150TAGplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAA 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
154778pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAGplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integration
154779pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAAplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integration
154780pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HAplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAA 263TAG, for transient transfection or stable, piggybac-mediated, integration
154781pAS_4xhyb PylT A41AA C55A CRFR1 95TAG 263TAA-HAplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAG 263TAA, for transient transfection or stable, piggybac-mediated, integration
154783pUC_h7SK Mma PylT (UUA)plasmid with single copy of Mma PylT (UUA anticodon)
154784pUC_h7SK M15 (CUA)plasmid with single copy of Mma PylT M15 mutant (CUA anticodon)
154785pUC_h7SK M15 (UUA)plasmid with single copy of Mma PylT M15 mutant (UUA anticodon)

Antibodies from Article