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pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HA
(Plasmid #154780)


Item Catalog # Description Quantity Price (USD)
Plasmid 154780 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • Mutation
    95TAA 263TAG in CRFR1-HA, hybrid PylT with A41AA and C55A mutation
  • Entrez Gene
    Crhr1 (a.k.a. CRF-R, CRF-R-1, CRF-R1, CRF1, CRFR-1, CRFR1, CRH-R, CRH-R 1, CRH-R-1, CRH-R1)
  • Promoter EF1
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

dual suppression of rat CRFR1 described by Serfling et al., 2018 (PMID: 29177436 )

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HA was a gift from Simon Elsaesser (Addgene plasmid # 154780 ; ; RRID:Addgene_154780)
  • For your References section:

    Site-Specific Incorporation of Two ncAAs for Two-Color Bioorthogonal Labeling and Crosslinking of Proteins on Live Mammalian Cells. Meineke B, Heimgartner J, Eirich J, Landreh M, Elsasser SJ. Cell Rep. 2020 Jun 23;31(12):107811. doi: 10.1016/j.celrep.2020.107811. 10.1016/j.celrep.2020.107811 PubMed 32579937