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(Plasmid #154786)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154786 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
    phage-ubc-nls-ha-tdMCP-gfp (Addgene Plasmid #40649)
  • Backbone size w/o insert (bp) 6200
  • Total vector size (bp) 9169
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
    Adenosine deaminse
  • Alt name
  • Species
    D. melanogaster (fly), Synthetic
  • Insert Size (bp)
  • Mutation
    Synonymized serine to prevent autoediting of ADAR, hyperediting mutation E488Q of ADAR
  • Entrez Gene
    Adar (a.k.a. Dmel_CG12598, ADAR, ADAR1, CG12598, Dmel\CG12598, EG:BACN35H14.1, adar, adr, cg12598, dADAR, dAdar, dadar, hypnos-2)
  • Promoter UBC
  • Tags / Fusion Proteins
    • mCherry (N terminal on insert)
    • V5 (C terminal on insert)
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (destroyed during cloning)
  • 3′ cloning site SphI (not destroyed)
  • 5′ sequencing primer UBC_F (5'-CGCCGATGATTATATAAGGA-3')
  • 3′ sequencing primer GFP_R (5'-GCATCACCTTCACCTTCACC-3')
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP was a gift from Robert Singer (Addgene plasmid # 154786 ; ; RRID:Addgene_154786)
  • For your References section:

    MS2-TRIBE Evaluates Both Protein-RNA Interactions and Nuclear Organization of Transcription by RNA Editing. Biswas J, Rahman R, Gupta V, Rosbash M, Singer RH. iScience. 2020 Jun 28;23(7):101318. doi: 10.1016/j.isci.2020.101318. 10.1016/j.isci.2020.101318 PubMed 32674054