PurposeEpisomally expresses KLF2 and NANOG in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||154879||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer agagcctctgctaaccatgt
- 3′ sequencing primer ctattgcaatgaaaataaatttcctttatt (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCE-K2N was a gift from Hideyuki Okano (Addgene plasmid # 154879 ; http://n2t.net/addgene:154879 ; RRID:Addgene_154879)
For your References section:Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts of Multiple Mammalian Species. Yoshimatsu S, Nakajima M, Iguchi A, Sanosaka T, Sato T, Nakamura M, Nakajima R, Arai E, Ishikawa M, Imaizumi K, Watanabe H, Okahara J, Noce T, Takeda Y, Sasaki E, Behr R, Edamura K, Shiozawa S, Okano H. Stem Cell Reports. 2021 Mar 23. pii: S2213-6711(21)00132-6. doi: 10.1016/j.stemcr.2021.03.002. 10.1016/j.stemcr.2021.03.002 PubMed 33798453