PurposeMiddle entry vector containing QF2w
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||155117||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2544
- Total vector size (bp) 3889
Vector typeGateway middle entry vector
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1345
- Cloning method Restriction Enzyme
- 5′ cloning site SacII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CAC GAC GTT GTA AAA CGA C
- 3′ sequencing primer CAG GAA ACA GCT ATG AC (Common Sequencing Primers)
Can be recombined with any p5E vector containing a promoter to allow for tissue-specific expression of QF2w. Note that an SV40pA is included after QF2w. QF2w originally described here (PMID: 25581800).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pME-QF2w-SV40pA was a gift from Bret Pearson (Addgene plasmid # 155117 ; http://n2t.net/addgene:155117 ; RRID:Addgene_155117)
For your References section:An optimized QF-binary expression system for use in zebrafish. Burgess J, Burrows JT, Sadhak R, Chiang S, Weiss A, D'Amata C, Molinaro AM, Zhu S, Long M, Hu C, Krause HM, Pearson BJ. Dev Biol. 2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 10.1016/j.ydbio.2020.07.007. 10.1016/j.ydbio.2020.07.007 PubMed 32697972