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(Plasmid #155165)


Item Catalog # Description Quantity Price (USD)
Plasmid 155165 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    self assembled
  • Backbone size w/o insert (bp) 3060
  • Total vector size (bp) 7097
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Use E. coli BL21(DE3) for expression and grow at room temperature.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Sucrose Phosphorylase from Bifidobacterium adolescentis
  • Alt name
  • Species
    Bifidobacterium adolescentis
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    BAD_RS00415 (a.k.a. BAD_RS00415, BAD_0078)
  • Tag / Fusion Protein
    • Strep - Tag II (N terminal on backbone)

Gene/Insert 2

  • Gene/Insert name
    Cellobiose phosphorylase from Cellulomonas uda
  • Alt name
  • Species
    Cellulomonas uda
  • Insert Size (bp)
  • GenBank ID
  • Tag / Fusion Protein
    • His Tag (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBICI_strong was a gift from Bernd Nidetzky (Addgene plasmid # 155165 ; ; RRID:Addgene_155165)
  • For your References section:

    Plasmid Design for Tunable Two-Enzyme Co-Expression Promotes Whole-Cell Production of Cellobiose. Schwaiger KN, Voit A, Dobiasova H, Luley C, Wiltschi B, Nidetzky B. Biotechnol J. 2020 Jul 15:e2000063. doi: 10.1002/biot.202000063. 10.1002/biot.202000063 PubMed 32668097