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Plasmid Design for Tunable Two-Enzyme Co-Expression Promotes Whole-Cell Production of Cellobiose.
Schwaiger KN, Voit A, Dobiasova H, Luley C, Wiltschi B, Nidetzky B
Biotechnol J. 2020 Jul 15:e2000063. doi: 10.1002/biot.202000063.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
155163pBICI_weakBaSP and CuCBP co-expression: weak RBS controls BaSP expression
155164pBICI_mediumBaSP and CuCBP co-expression: medium RBS controls BaSP expression
155165pBICI_strongBaSP and CuCBP co-expression: strong RBS controls BaSP expression
155167pBICI_2_mediumsee pBICI_medium: exchanged origin of replication (pBR322) and introduced bom site
155168pBICI_2_strongsee pBICI_strong: exchanged origin of replication (pBR322) and introduced bom site
155169pDUAL_lacZBaSP and CuCBP co-expression: lacZ promoter controls BaSP expression
155170pDUAL_tacIBaSP and CuCBP co-expression: tacI promoter controls BaSP expression
155172pDUAL_araBADBaSP and CuCBP co-expression: araBAD promoter controls BaSP expression
155173pDUAL_2_lacZsee pDUAL_lacZ: exchanged origin of replication (pBR322) and introduced bom site
155174pDUAL_2_tacIsee pDUAL_tacI: exchanged origin of replication (pBR322) and introduced bom site
155175pDUAL_2_T5see pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom site
155176pDUAL_2_araBADsee pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom site
155178pBR322-bompBICI_2_strong without bom site

Antibodies from Article