pcDNA3-HA-DNM2-R465W
(Plasmid #155262)

• Purpose: Mammalian expression of rat DNM2 fused to HA
• Depositing Lab: David Rubinsztein
• Publication: Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6. doi: 10.1016/j.devcel.2020.03.018.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dynamin 2
• Species: R. norvegicus (rat)
• Mutation: Arginine 465 to Tryptophan
• Entrez Gene: Dnm2 (a.k.a. DYIIAAB)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: dynamin 2 (mutated from Addgene Plasmid #34684)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry