pJK430
(Plasmid #155377)

• Purpose: PphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus]
• Depositing Lab: Pamela Silver
• Publication: Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):8165-8177. doi: 10.1093/nar/gkaa557.

Backbone

• Vector backbone: SC101
• Total vector size (bp): 10815
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: dCas9 (bacteria)
• Alt name: deactivated Cas9 (S. pyogenes)
• Species: S. pyogenes
• Insert Size (bp): 4107
• Mutation: D10A H840A (catalytically inactive)

Gene/Insert 2

• Gene/Insert name: PA4-mVenus
• Insert Size (bp): 997

Gene/Insert 3

• Gene/Insert name: PphlF_A2NT in dCas9 sgRNA oscillator v1
• Species: Synthetic
• Insert Size (bp): 1557

Resource Information

• Supplemental Documents:
  • pJK430_PAS.gb
• A portion of this plasmid was derived from a plasmid made by: pdCas9-bacteria (Stanley Qi, Addgene #44249); pLPT119 (Johan Paulsson, Addgene #85526); Christopher Voigt (Addgene #62307, 62308)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry