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Toward a translationally independent RNA-based synthetic oscillator using deactivated CRISPR-Cas.
Kuo J, Yuan R, Sanchez C, Paulsson J, Silver PA
Nucleic Acids Res. 2020 Aug 20;48(14):8165-8177. doi: 10.1093/nar/gkaa557.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
155369pJK384.4dCas9 + PA4-mVenus
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155370pJK398dCas9 sgRNA oscillator v1 (dCRv1) (p15A)
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155371pJK412dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]
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155372pJK413dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)
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155373pJK422dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)
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155374pJK423dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)
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155376pJK429PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]
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155377pJK430PphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus]
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155378pJK434PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)
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155380pJK503dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus
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155381pJK504dCas12a crRNAs
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155382pJK506dCas12a (D917A) + PA4-mVenus. (removed crRNAs)
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155384pJK511dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus
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155385pJK517PphlF-TA4 in crRNAs
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155386pJK520PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus
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155387pJK526PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus
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155388pJK528dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)
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