PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||156829||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6106
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1194
MutationFc region of human IgG contains D265A and P329A mutations (DAPA)
Entrez GeneHLA-A (a.k.a. HLAA)
- Promoter CMV/SP6
/ Fusion Protein
- Fc(DAPA)-AviTag-6xHis (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site GCGGCCGC (not destroyed)
- 3′ cloning site GGCGCGCC (not destroyed)
- 5′ sequencing primer ATTTAGGTGACACTATAG
- 3′ sequencing primer CACGTACCAGTTGAACTTCACC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bysynthesis by ThermoFisher
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Some plasmids in this collection may have an IS4-like element ISVsa5 family transposase upstream of the CMV promoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pD649-HAsp-HLA-A-Fc(DAPA)-AviTag-6xHis was a gift from Chris Garcia (Addgene plasmid # 156829 ; http://n2t.net/addgene:156829 ; RRID:Addgene_156829)
For your References section:A Human IgSF Cell-Surface Interactome Reveals a Complex Network of Protein-Protein Interactions. Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC. Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 10.1016/j.cell.2020.07.025. 10.1016/j.cell.2020.07.025 PubMed 32822567